The Determination of Neutral Fat Glycerol in Blood with Periodate
نویسنده
چکیده
Recent work (1) on the blood precursors of milk fat, by attaching special significance to the triglycerides of blood in the formation of milk, has illustrated forcefully the need of a more direct method for the quantitative evaluation of neutral fat in blood. Until Kelsey (2) reported the use of lipases, the isolation of the glyceride fatty acids had not been accomplished because of the homologous nature of the fatty acids in the acetone-soluble lipids of blood. The only alternative for the direct estimation of neutral fat is the determination of glycerol after saponification of the acetonesoluble lipids. A method suitable for this purpose is proposed herein. Its application to the determination of arteriovenous differences in neutral fat of the blood of the lactating cow is described. The method is based on the reaction of periodic acid and periodates on polyhydric alcohols or acids having adjacent hydroxyl groups. This reaction was discovered and its applications described by Malaprade (3). Glycerol reacts with periodate in acid solution according to the equation
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